摘要：Metallo-beta-lactamases (MBLs)-producing Gram-negative bacilli (GNB) are one of the significant multidrug-resistant pathogens causing healthcare-associated infection (HAI) worldwide.The present study aimed to compare the phenotypic and molecular methods to detect MBLs-producing GNB causing HAI,namely Acinetobacter baumannii,Escherichia coli,Klebsiella pneumoniae,and Pseudomonas aeruginosa in a tertiary care hospital.Materials and Methods: Antimicrobial susceptibilities were determined for 232 isolates identified during 8 months period,and the Modified Hodge Test confirmed carbapenemases production in carbapenem-resistant isolates as per the Clinical and Laboratory Standards Guidelines 2013.MBLs production was detected by the disc enhancement test (DET),combined disk test (CDT),and polymerase chain reaction (PCR) and statistically analyzed.Results: One hundred (43.1%) isolates were found to be carbapenem-resistant,of which 85% were positive on the Modified Hodge Test.The DET was 96.67%,whereas the CDT was 93.33% sensitive as compared to the PCR.Kappa coefficient ranged from 0.918 to 0.959 for different methods indicating the excellent agreement between the three methods.Conclusion: The DET and CDT showed excellent agreement with molecular methods to detect MBLs-producing isolates.They are easy to perform,inexpensive,and can be routinely used in clinical laboratories.
