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  • 标题:Development of Pretreatment Protocols for Determination of Soybean β-Conglycinin in Processed Soybean Foods Using Commercial ELISA Kits
  • 本地全文:下载
  • 作者:Tatsuya MORIYAMA ; Erika YANO ; Nobuhiro ZAIMA
  • 期刊名称:Journal of Nutritional Science and Vitaminology
  • 印刷版ISSN:0301-4800
  • 电子版ISSN:1881-7742
  • 出版年度:2020
  • 卷号:66
  • 期号:3
  • 页码:270-277
  • DOI:10.3177/jnsv.66.270
  • 出版社:Center for Academic Publications Japan
  • 摘要:β-Conglycinin is the major storage protein in soybeans. Pre-clinical animal models and human clinical studies have demonstrated the triglyceride-lowering effect of this protein, suggesting that it could be put into practical use as a functional food material. To date, however, there are no accurate and simple assays for quantification of β-conglycinin. In this study, samples were pretreated by mixing them with rice flour powder prior to extraction of proteins. Then, we used commercially available ELISA kits for detection of allergens that could be present in any contaminating soybean residue. This enabled accurate and highly reproducible quantitation of β-conglycinin content in several processed soybean foods.
  • 关键词:β-conglycinin;ELISA;assay;soybean;Kori-tofu;soymilk
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