摘要：Objective: This study aimed to identify the phytochemical compounds of the ethanolic stem extract of Cleome gynandra (CGEE) and to determine the antioxidant, membrane stability and analgesic activities of CGEE . Materials and Methods: Phyto chemical analysis was conducted qualitatively. Content of total phenolic compounds and tannins was determined using Folin Ciocalteau reagent. Antioxidant activity was evaluated using the 1,1-diphenyl-2-picrylhydrazyl assay, membrane stabilizing activity was tested using hypotonic solution and heat-induced hemolysis and analgesic activities were evaluated using the acetic acid-induced writhing test in mice. Results: CGEE is rich in tannins, flavonoids, steroids, glycosides, acidic compounds and proteins. The total phenolic and tannin contents were 237.92 mg gallic acid equivalents (GAE)/100 g and 21.07 mg GAE/100 g of dried plant extract, respectively. CGEE also displayed promising DPPH free radical scavenging activity, with an IC50 value of 9.62 μg mL1. In the hypotonic solution-induced hemolysis test, the extract showed 43.67, 42.85 and 38.66% inhibition at 0.5, 1.0 and 2.0 mg mL1, respectively, whereas the standard resulted in 30.57% inhibition. In the heat-induced hemolysis test, 1 mg mL1 of extract resulted in 84.43% inhibition of hemolysis. Furthermore, in the analgesic activity test, CGEE doses of 250 and 500 mg kg1 body weight resulted in good inhibition of the writhing reflex (40.87 and 53.92%, respectively), while the standard drug (diclofenac sodium) at 25 mg kg1 body weight resulted in inhibition of 64.35%. Conclusion: Results of this study suggest that the stem extract of Cleome gynandra possesses antioxidant, membrane stabilizing and analgesic activities.
