Abstract Ensuring the quality of cycling infrastructure is one of the ways to promote the cycling accessibility, which contributes to a more sustainable urban mobility. The aim of this paper is to elaborate a Quality Assessment Index of Cycling Infrastructures and apply it for analyzing the cycling accessibility conditions of the existing cycle paths and cycle lanes in João Pessoa. The methodology corresponds to a bibliographic research about methods of quality evaluation of bicycle systems, as a parameter for the development of the proposed index, continuing with a field research and data collection necessary for the application. The results contribute to a mapping and diagnosis of the current situation of João Pessoa’s cycle structure. In addition, to providing new methodological parameters of analysis through the elaboration and application of the index, composed by 4 categories and 12 indicators. It reveals a series of vulnerabilities regarding the conditions of the cycle paths and cycle lanes, especially regarding the signaling, demonstrating the need for intervention according to the performance of the evaluation, and thus contributing to planning actions.