ABSTRACT Purpose To investigate the immediate effect of phonation into silicone tube on self-assessment and vocal quality of gospel singers. Methods The sample was composed of 40 gospel singers without vocal complaint, males and females, from 18 to 40 years. The singers performed semi-occluded vocal tract exercise in LaxVox® silicone tube. The end of the tube was submerged in water (2cm depth), for three minutes. Before and after exercise, voice recording was performed for perceptive-auditory evaluation, and only after exercise, the voice and comfort phonation self-assessments (CSA) were performed. The descriptive and inferential statistical analysis were performed. Results All participants reported improvement in voice. In addition, a significant higher proportion of them indicated phonation comfort improvement after exercise. According to the perceptive-auditory evaluation, there was no difference in the comparison of the proportions of pre- and post-exercise perceptive-auditory evaluation categories in gospel singers. Conclusion The phonation exercise into a silicone tube caused an immediate positive effect on gospel singers’ voice and comfort phonation self-assessments. There was no significant difference in the perceptive-auditory evaluation before and after phonation into silicone tube in gospel singers.