期刊名称：Brazilian Journal of Forensic Sciences, Medical Law and Bioethics
电子版ISSN：2237-261X
出版年度：2016
卷号：5
期号：4
页码：410-430
DOI：10.17063/bjfs5(4)y2016410
出版社：Instituto Paulista de Estudos Bioéticos e Jurídicos
摘要：Article 158 of the Brazilian Criminal Procedure Code (BCPC) provides that when the infringement lets trace elements, it will be essential the examination of the corpus delicti.However, many fingerprints that arrive to confrontation at the Technical and Scientific Police of Goiás (SPTC) do not present conditions of analysis, being blurred or incomplete.For this reason, this study aimed to: analyze fingerprints by optical microscopy, verifying the presence of epidermal cells; extract DNA from latent fingerprints attached to different surfaces and those developed with spray of Ninhydrin or impregnated with black powder volcano HI-FI of SIRCHIE.This study has detected scaly cells in 98% of the slides stained with Leishman.In another experiment, DNA molecules were extracted from 69 samples deposited in five different surfaces (aluminium, wood, paper, plastic and glass), resulting in DNA concentrations that varied between 0.3 ng/ µ L to 25.4 ng/ µ L.Particularly, wood was the surface with the highest mean DNA concentration (10.67 ng/µ L).DNA samples extracted from fingerprints, that were revealed with Ninhydrin or impregnated with black powder, were amplified in 70% and 60% of the cases, respectively.The present work corroborates with several studies that have demonstrated the possibility of extracting DNA from surfaces that have simply been touched by the hands of a person.The experiments also demonstrated that higher concentrations of DNA were obtained from porous surfaces comparing to smooth surfaces, and that the use of Ninhydrin and black powder allows efficient extraction of the genetic material.
关键词：Fingerprint; Surface; DNA; Cells; Ninhydrin; Black powder
