期刊名称：Brazilian Journal of Forensic Sciences, Medical Law and Bioethics
电子版ISSN：2237-261X
出版年度：2017
卷号：7
期号：1
页码：69-79
DOI：10.17063/bjfs7(1)y201769
出版社：Instituto Paulista de Estudos Bioéticos e Jurídicos
摘要：Blood can be found in various forms at crime sites: clotted, dried, stained, smeared and deposited on different surfaces.The detection and confirmation of blood with laboratory tests helps the scientific police in the identification of the criminal, victim, and the in the comprehension of the dynamics of facts.The Feca Cult One Step, a specific test for detecting human hemoglobin, is very useful in the laboratory and also for crime scenes because it is a practical and fast analysis.Due to the high sensitivity of this test, the research was developed with the purpose of evaluating if any factor could interfere in the result and affect the elucidation of a crime.For the technique, it was collect venous blood from four donors.Then donated blood was deposited on twenty-four different substrates (beet, red powder gelatin, artificial juice in powder, purple earth, soil, wood, asphalt, coins (R$ 0.05, 0.10, 0.25, 0.50 and 1.00), black tea with cinnamon, green tea, beef, leaf, sand, straw smoke, dark denim fabric, rust, plastic with magnet, hypochlorite, detergent and bleach).Due to the accuracy of the immunochromatographic test, the results were compatible with the expected results.However, some tests presented false-negative and false-positive results.In addition to these results that impair performance of the test, technical errors at the time of sample collection, prolonged contact with chemical agents, and excessive dilution of blood spots are interferences that should be considered when using Feca Cult One Step on identification of human blood.
