出版社：Polish Society of Ecological Engineering (PTIE)
摘要：Fluoride is regarded as one of the strongest oxidants, which causes oxidative changes in cells of living organisms.It may both increase the content of reactive oxygen species and inhibit the activity of antioxidative enzyme.In recent years, many researchers successfully used the properties of clay minerals in the sorption of fluoride ion from water.This raises the question of the possibility of limiting the effect of fluorine on the negative changes in plants by adding bentonite to soil.A two-year pot experiment was carried out in the Greenhouse of West Pomeranian University of Technology in Szczecin, on loamy sand and sandy loam.Each sample of soil was mixed with three different concentrations of bentonite – 1, 5, 10% of dry weight (DW) of the soil and then treated with 30 mmol of F- per 1 kg of dry weight of the soil in a form of NaF solution.A control series was prepared for each soil, to which no additives were added.The medium prepared in such way was transferred to plastic pots (3 kg each) and seeded with 16 pea seeds of Pisum sativum.In three phases of pea development (4 leaves unfolded, flowering and development of fruit), fresh leaf samples were collected and the concentrations of ascorbic acid, reduced glutathione, total flavonoids and total polyphenols were measured.Sodium fluoride introduced to the soil changed the level of antioxidant parameters in the plant, which may suggest that fluoride is involved in the formation of reactive oxygen species, resulting in oxidative stress.Bentonite in a dosage of 10% reduced the toxic effects of fluoride on the oxidative balance and morphological changes in the plant, which was observed especially for loamy sand, naturally poor in clay minerals.
