出版社：Polish Society of Ecological Engineering (PTIE)
摘要：The aim of the study was to evaluate the usefulness of Escherichia coli K-12 RFM 443 recA::lux for cytotoxicity and genotoxicity monitoring of metoprolol in the environment.Metoprolol is one of the most popular cardiac drug which belongs to the group of β-blockers.The drug was applied at the concentrations ranging from 0.01 µg/cm3 to 100 µg/cm3.The conducted research constitutes preliminary study aimed at validation of the recA::lux gene construct in order to determine its sensitivity to metoprolol.The drug concentrations were selected experimentally to obtain a positive luminescence response.The obtained data indicated the influence of metoprolol on lux gene expression and recA promoter activity based on the application of laboratory samples using PBS buffer.The results indicate a potential for using a bacterial biosensor Escherichia coli K-12 RFM 443 with recA::lux gene fusion in cytotoxicity and genotoxicity monitoring of the cardiac drugs residue in the environment.
