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  • 标题:Combined Exposure of ELF Magnetic Fields and X-rays Increased Mutant Yields Compared with X-rays Alone in pTN89 Plasmids
  • 其他标题:Combined Exposure of ELF Magnetic Fields and X-rays Increased Mutant Yields Compared with X-rays Alone in pTN89 Plasmids
  • 本地全文:下载
  • 作者:Shin KOYAMA ; Takehisa NAKAHARA ; Tomonori SAKURAI
  • 期刊名称:Journal of Radiation Research
  • 印刷版ISSN:0449-3060
  • 电子版ISSN:1349-9157
  • 出版年度:2005
  • 卷号:46
  • 期号:2
  • 页码:257-264
  • DOI:10.1269/jrr.46.257
  • 摘要:We have examined mutations in the supF gene carried by pTN89 plasmids in Escherichia coli ( E. coli ) to examine the effects of extremely low frequency magnetic fields (ELFMFs) and/or X-rays to the plasmids. The plasmids were subjected to sham exposure or exposed to an ELFMF (5 mT), with or without X-ray irradiation (10 Gy). For the combined treatments, exposure to the ELFMF was immediately before or after X-ray irradiation. The mutant fractions were 0.94 × 10 -5 for X-rays alone, 1.58 × 10 -5 for an ELFMF followed by X-rays, and 3.64 × 10 -5 for X-rays followed by an ELFMF. Increased mutant fraction was not detected following exposure to a magnetic field alone, or after sham exposure. The mutant fraction for X-rays followed by an ELFMF was significantly higher than those of other treatments. Sequence analysis of the supF mutant plasmids revealed that base substitutions were dominant on exposure to X-rays alone and X-rays plus an ELFMF. Several types of deletions were detected in only the combined treatments, but not with X-rays alone. We could not find any mutant colonies in sham irradiated and an ELFMF alone treatment, but exposure to ELFMFs immediately before or after X-ray irradiation may enhance the mutations. Our results indicate that an ELFMF increases mutation and alters the spectrum of mutations.
  • 关键词:Extremely low frequency magnetic fields; X-rays; Mutation; pTN89 plasmids
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