摘要：We have studied the effects of 2-deoxy-D-Glucose (2-DG) on the recovery of potentially lethal damage (PLDR), repair of chromosomal DNA, cyclobutane pyrimidine dimers (CPDs), reverse mutation and gene-conversion in UVC (254 nm) irradiated yeast. As studied by pulsed-field gel electrophoresis, post-irradiation chromosomal DNA repair kinetics in a phosphate buffer (PB) with 10 m M glucose (G) was biphasic, where the first phase exhibited a decrease and the second phase showed an increase in the band intensities. A post-irradiation treatment in PB G (10 m M ) with 2-DG (10, 20, 50 m M ) reduced the decrease in the DNA band intensities in the first phase of DNA repair. As compared to a post-irradiation (125 J/m 2 ) treatment in PB G (10 m M ), a treatment in PB G (10 m M ) 2-DG (10 m M ) showed a decreased PLDR, but increased revertants and gene-convertants.
