标题：Identification of a New G-to-A Transition Mutation at Nucleotide Position 129 of the Xrcc4 Gene in Ionizing Radiation-hypersensitive Mutant LX830 Cells
其他标题：Identification of a New G-to-A Transition Mutation at Nucleotide Position 129 of the Xrcc4 Gene in Ionizing Radiation-hypersensitive Mutant LX830 Cells
摘要：The mouse lymphoma cell line LX830 is an X-ray-hypersensitive mutant. Complementation tests between LX830 cells and radiation-sensitive mutants of M10 ( Xrcc4 deficient cells) or SX10 ( DNA ligase IV deficient cells) cells showed that M10 cells did not complement LX830 cells, but SX10 cells did, suggesting that LX830 cells would belong to the X-ray-cross complementation group (XRCC4). A sequence analysis of Xrcc4 cDNA in LX830 cells disclosed a transition of G to A at nucleotide position 129, which resulted in a change of tryptophan (43) to a termination codon. Transfection of the mouse Xrcc4 cDNA rescued the X-ray sensitivity of the mutant cells. LX830 is an Xrcc4 -deficient cell line bearing a termination codon in exon 2 of the Xrcc4 gene and no wild-type Xrcc4 gene.
