出版社：Brazilian Association of Sleep and Latin American Federation of Sleep Societies
摘要：Introduction Obstructive sleep apnoea (OSA) is common in obesity and is associated with cardiovascular and metabolic complications. Continuous positive airway pressure (CPAP) in {OSA} may lead to physiological changes reflected in the urinary proteome. The aim of this study was to characterise the urinary proteome in severely obese adult subjects with {OSA} who were receiving {CPAP} compared with severely obese subjects without OSA. Methods Severely obese subjects with and without {OSA} were recruited. Subjects with {OSA} were receiving CPAP. Body composition and blood pressure measurements were recorded. Urinary samples were analysed by Capillary Electrophoresis–Mass Spectrometry (CE–MS). Results Twenty-seven subjects with OSA-on-CPAP (age 49±7years, {BMI} 43±7 kg/m2) and 25 controls without {OSA} (age 52±9years, {BMI} 39±4 kg/m2) were studied. Age and {BMI} were not significantly different between groups. Mean {CPAP} use for {OSA} patients was 14.5±1.0 months. Metabolic syndrome was present in 14(52%) of those with {OSA} compared with 6(24%) of controls (p=0.039). A urinary proteome comprising 15 peptides was identified showing differential expression between the groups (p<0.01). Although correction for multiple testing did not reach significance, sequences were determined for 8 peptides demonstrating origins from collagens, fibrinogen beta chain and T-cadherin that may be associated with underlying cardiovascular disease mechanisms in OSA. Conclusions The urinary proteome is compared in {OSA} with {CPAP} and without {OSA} in severe obesity. The effects of {CPAP} on {OSA} may lead to changes in the urinary peptides but further research work is needed to investigate the potential role for urinary proteomics in characterising urinary peptide profiles in OSA.
