摘要：The study aimed to investigate the effects of the exercise training on inflammatory gene expression in adipose tissue via aging and obesity. METHODS The study subjects were forty 10-week old and 70-week old male Wistar rats. The young and old-aged rats were divided into four groups of ten: control (non-exercise) group (Y-Non; O-Non) and exercise group (Y-Ex; O-Ex). After the exercise training, intergroup differences in body composition, oral glucose tolerance test result, and protein expression levels of IL-6, TNFα, IL-10, and IL-4 in adipose tissue, were compared. RESULTS Both body weight and body fat-free mass were lower in old-aged rats than young rats; O-Non group, in particular, displayed the highest body fat mass and lowest fat-free mass. In oral glucose tolerance test result, old-aged rats exhibited higher blood glucose and insulin concentrations than young rats. TNF-α protein expression was higher in old-aged rats than young rats with the Y-Ex group displaying the lowest expression. IL-6 protein expression was significantly lower in the exercise group than in the control group for both old-aged and young rats (p < 0.05). IL-10 protein expression was not significantly different between the exercise group and the control group for either old-aged or young rats. IL-4 protein expression was significantly lower in the exercise group of young rats (p < 0.05). CONCLUSIONS Based on these findings, the analysis of the effects of exercise training on inflammatory gene expression in the adipose tissue of obese old-aged rats showed that the exercise training has effectively promoted the shift to M2 of cytokines secretion pattern related to obesity-induced pro-inflammation in old-aged rats and helped to improve insulin sensitivity.
