摘要:S U M M A R Y Background In clinical routine, preoperative radiographic assessment of lower extremity geometry relies on conventional X-rays. However, the plane goniometric measuring has several limitations in accurately locating anatomical landmarks. The purpose of this study is to propose a fast and accurate 3D-reconstruction-method based on biplanar X-rays with clinical measurements assessment in standing position. Methods 50 candidates for HTO or DFO with deformity of the lower extremities were included in this study. Biplanar X-rays were performed using the EOS imaging system in conventional double-stance full weight-bearing position (DS) and shifted-foot standing position (SF). The results of hip-knee-ankle angle (HKAA), lateral distal femoral angle (LDFA), and medial proximal tibial angle (MPTA) were evaluated by either 2D manual goniometer (MG) based on X-ray in DS standing position or 3D-reconstruction goniometer based on X-rays in SF standing position. Results For the reproducibility study, MG and EOS goniometer were both reliable in repeated measures of HKAA, LDFA, and MPTA, with average concordance correlation coefficients (CCCs) all above 0.910. The agreements between MG and EOS measurements were high for HKAA and LDFA with CCCs all above 0.90, while the agreement was low for MPTA with CCC below 0.75. Further linear regression model analysis also revealed a significant correlation between MG and EOS measurements for HKAA (all R2 ≥ 0.93) and LDFA (all R2 ≥ 0.90), but not for MPTA (all R2 ≤ 0.522). Conclusion In comparison with the traditional 2D manual goniometer, EOS 3D reconstruction based goniometric measuring could provide equivalent results of HKAA and LDFA, and potentially a more accurate result of MPTA. These findings suggest that EOS 3D reconstruction based goniometric measuring is suitable for preoperative evaluation and planning for HTO/DFO. However, future improvements of the 3D reconstruction method are needed for better detection of the femoral condyles and tibial plates without the requirement of shifted-foot standing position. The translational potential of this article EOS 3D reconstruction based goniometric measuring could provide equivalent or even more accurate results of HKAA, LDFA, and MPTA, in comparison with the traditional 2D manual goniometer, making it suitable for preoperative evaluation and planning for HTO/DFO.