As a sportification form of human-computer interaction, eSports is facing great gender stereotype threat and causing female players’ withdraw. This study aims to investigate the relationship between gender-swapping and females’ continuous participation intention in eSports, the mediating effect of self-efficacy, and the moderating effect of discrimination. The results demonstrate (1) that the effect of gender-swapping on continuous participation intention in eSports was not significant, while gender-swapping had a significant association with self-efficacy, and self-efficacy had a significant association with continuous participation intention in eSports; (2) that gender-swapping had an indirect effect (via self-efficacy) on continuous participation intention in eSports; and (3) that discrimination moderated the effect of self-efficacy on continuous participation intention. Female players who had experienced discrimination displayed higher continuous participation intention in the context of self-efficacy enhanced by gender-swapping.