摘要：Continuous cropping of tomato is increasingly practiced in greenhouse cultivation, leading to several soil-related obstacles. In this study, a type of microbial restoration substrate (MRS) was used to amend soils from the re-cropping of tomato for 8 years under greenhouse-cultivated conditions. Two treatments were established: using 1,500 kg hm−2 of MRS to amend soil as treatment (TR), and non-MRS as control (CK). The severity of bacterial wilt (BW), soil properties and rhizobacterial community composition under two different treatments were compared. The application of MRS led to an average 83.75% reduction in the severity of BW, and significantly increased the plant height, root activity and yield. Meanwhile, soil pH, soil organic contents (SOC), total nitrogen (TN) and exchangeable calcium were significantly increased (P < 0.05) by MRS treatment. Illumina-MiSeq sequencing analysis of the 16S rRNA genes revealed that MRS increased the diversity of the tomato rhizobacterial community. The relative abundances of Proteobacteria, Actinobacteria and Bacteroidetes were enhanced, whereas those of Acidobacteria, Chloroflexi, TM7 and Firmicutes were decreased by MRS. The redundancy analysis (RDA) revealed that the severity of tomato BW was negatively correlated with the relative abundances of Actinobacteria, Bacteroidetes and Proteobacteria, but positively correlated with those of Gemmatimonadetes, Firmicutes and Acidobacteria. In addition, the effects of MRS on rhizobacterial metabolic potentials were predicted using a Kyoto Encyclopedia of Genes and Genomes (KEGG) database, implying that MRS could significantly increase nitrogen metabolisms and reduce carbon metabolism. Together, our results indicated that the use of MRS could reestablish soil microbial communities, which was beneficial to plant health compared with the control.
