出版社：Regional Information Center for Science and Technology (RICeST)
摘要：The aim of this study was to investigate the histopathologic changes of spleen tissues as biomarkers for assessing the health of common Pomadasys kaakan and Lutjanus johnii . In this research, 9 pieces of Pomadasys kaakan with a mean length 35.54 ± 4.35 cm and a mean weight of 512.6 ± 58.5 grams and 9 pieces of Lutjanus johnii with an average length of 22.5 ± 3.41 cm and mean weight 205.6 ± 22 grams of Ramin, Haft Tir and Konarak stations were caught using by gillnet. In order to observe the severity of tissue lesions, spleen tissue was separated and fixed in Bouinchr('39')s solution. The tissue samples were prepared for histological studies according to standard histology methods, dehydration, clarification, parafinization. Then they were prepared by microtome cutting device with transverse sections of 5 microns thickness. After staining with hematoxylin-eosin, it was examined by a light microscope equipped with a digital photographic Camera. Results showed lesions in the spleen tissue such as melanomacrophage aggregations, blood vessel dilatation, vacuolation, necrosis, cell density, white pulp, plasma cells dilution. Lowest lesions in the spleen tissue of Pomadasys kaakan and Lutjanus johnii were observed at Ramin and Konarak stations. In the other hand, the highest lesions in the spleen tissue of both fish were recorded at Hatf Tir station. Which can be explained by the close vicinity of the station and the proximity of two Shahid Kalantari and Shahid Beheshti docks and high levels traffic of flotation.
关键词：Tissue lesions ; Spleen ; Biomarker ; Chabahar Bay
