(Freshwater hyphomycetes in a preserved insular system from Brazil: first records at Parque Estadual da Ilha do Cardoso, municipality of Cananéia, São Paulo State). To survey freshwater hyphomycetes from insular water bodies at the Parque Estadual da Ilha do Cardoso State Park, in Cananéia municipality, São Paulo State, Brazil, submerged mixed leaf litter samples were collected from six sites from June 2012 to February 2013. The samples were taken to the laboratory and incubated in Petri dishes containing sterile distilled water at room temperature (20oC). From the 5th day on, until at least one month, fragments of the leaf litter samples were examined under the microscope, resulting in the morphological identification of 33 taxa. Among those, two are new records for the state of São Paulo and four for Brazil. Descriptions and pictures of each new record are presented.