标题：Immune responses and resistance of white shrimp (Litopenaeus vannamei) fed Probiotic Bacillus sp NP5 and prebiotic honey against White Spot Syndrome Virus infection
其他标题：Respons imun dan resistansi udang vaname Litopenaeus vannamei yang
diberi Bacillus sp. NP5 dan madu terhadap infeksi white spot
syndrome virus
摘要：White spot disease caused by White Spot Syndrome Virus (WSSV) is the most serious viral disease and has a major impact on the decline in production of white shrimp farm. Improving the immune response through the application of prebiotic, probiotic and synbiotic is expected to be one of the environmentally friendly alternatives to prevent the disease. This study aimed to evaluate the effect of administrating Baccillus sp. NP5 probiotic, honey prebiotic and a combination both (synbiotic) in enhancing immune response and resistence of white shrimp to WSSV infection. This study consisted of five treatments and three replications, namely positive control (feeding without probiotics, prebiotics, and synbiotics then challenged with WSSV), negative control (feeding without probiotics, prebiotics, synbiotics, then injected with PBS), pro (feeding with the addition of Bacillus sp. NP5 probiotics then challenged against WSSV), pre (feeding with the addition of honey prebiotics then challenged against WSSV), and sin (feeding with the addition of synbiotics then challenged against WSSV). White shrimp with an average weight of 1.8±0.06 gram/shrimp were reared at a density of 15 shrimps per aquarium (60 cm x 30 cm x 30 cm and water height of 20 cm) for eight weeks, then challenge against WSSV at lethal dose (LD 50 ) dose as much as 0.1 ml per shrimp. The results showed that the immune response and resistence of white shrimp after treatments of probiotics, prebiotics, and synbiotics were better than those in controls with the optimal found in prebiotics treatment.
其他摘要：White spot disease yang disebabkan oleh white spot syndrome virus (WSSV) merupakan penyakit viral yang
berdampak besar terhadap penurunan produksi budidaya udang vaname Litopenaeus vannamei. Perbaikan respons
imun melalui aplikasi probiotik, prebiotik, dan sinbiotik dapat menjadi salah satu alternatif untuk pencegahan
serangan penyakit tersebut. Penelitian ini bertujuan mengevaluasi pengaruh pemberian Bacillus sp. NP5, madu dan
gabungan keduanya (sinbiotik) dalam meningkatkan respons imun dan resistansi udang vaname terhadap infeksi
WSSV. Penelitian ini terdiri dari lima perlakuan dan tiga ulangan yaitu kontrol positif (pemberian pakan tanpa
probiotik, prebiotik, dan sinbiotik kemudian diuji tantang dengan WSSV), kontrol negatif (pemberian pakan tanpa
probiotik, prebiotik, dan sinbiotik kemudian diinjeksi phosphate buffer saline), pro (pemberian pakan dengan
penambahan Bacillus sp. NP5 kemudian diuji tantang dengan WSSV), pre (pemberian pakan dengan penambahan
madu kemudian diuji tantang dengan WSSV), dan syn (pemberian pakan dengan penambahan sinbiotik kemudian
diuji tantang dengan WSSV). Udang vaname dengan bobot rata-rata 1.8 ± 0.06 g/ekor dipelihara dengan kepadatan
15 ekor per akuarium selama delapan minggu, kemudian diuji tantang dengan WSSV pada lethal dose 50% (LD50)
10-4 dengan volume 0.1 mL per ekor. Hasil penelitian ini menunjukkan perlakuan prebiotik menunjukkan hasil
yang lebih tinggi pada respons imun dan resistansi udang vaname dibanding perlakuan kontrol, probiotik, dan
prebiotik.
