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  • 标题:Electrochemical Immunosensors Based on Screen-Printed Gold and Glassy Carbon Electrodes: Comparison of Performance for Respiratory Syncytial Virus Detection
  • 本地全文:下载
  • 作者:Wioleta Białobrzeska ; Daniel Firganek ; Maciej Czerkies
  • 期刊名称:Biosensors
  • 电子版ISSN:2079-6374
  • 出版年度:2020
  • 卷号:10
  • 期号:11
  • 页码:175-187
  • DOI:10.3390/bios10110175
  • 出版社:MDPI Publishing
  • 摘要:This paper presents the development and comparison of label-free electrochemical immunosensors based on screen-printed gold and glassy carbon (GC) disc electrodes for efficient and rapid detection of respiratory syncytial virus (RSV). Briefly, the antibody specific to the F protein of RSV was successfully immobilized on modified electrodes. Antibody coupling on the Au surface was conducted via 4-aminothiophenol (4-ATP) and glutaraldehyde (GA). The GC surface was modified with poly-L-lysine (PLL) for direct anti-RSV conjugation after EDC/NHS (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-Hydroxysuccinimide) activation. Electrochemical characterizations of the immunosensors were carried out by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). GC-based immunosensors show a dynamic range of antigen detection from 1.0 × 105 PFU/mL to 1.5×107 PFU/mL, more than 1.0 × 105 PFU/mL to 1.0 × 107 PFU/mL for the Au-based sensor. However, the GC platform is less sensitive and shows a higher detection limit (LOD) for RSV. The limit of detection of the Au immunosensor is 1.1 × 103 PFU/mL, three orders of magnitude lower than 2.85 × 106 PFU/mL for GC. Thus, the Au-based immunosensor has better analytical performance for virus detection than a carbon-based platform due to high sensitivity and very low RSV detection, obtained with good reproducibility.
  • 关键词:respiratory syncytial virus; cyclic voltammetry; electrochemical impedance spectroscopy; sensor; gold electrode; glassy carbon respiratory syncytial virus ; cyclic voltammetry ; electrochemical impedance spectroscopy ; sensor ; gold electrode ; glassy carbon
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