标题：Upregulation and stabilization of senescence marker protein-30 by epigallocatechin gallate against tert-butyl hydroperoxide-induced liver injury in vitro and in vivo
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：2021
卷号：68
期号：1
页码：51-57
DOI：10.3164/jcbn.20-119
出版社：The Society for Free Radical Research Japan
摘要：Senescence marker protein-30 (SMP30), a novel ageing marker, suppresses oxidative stress in the liver. However, studies on phytochemical-mediated regulation of SMP30 expression are lacking. Here, we showed that epigallocatechin gallate (EGCg), a polyphenol abundant in green tea, positively regulates SMP30 expression in the rat hepatoma-derived Fao cells. EGCg maintained SMP30 expression even in the presence of cycloheximide, a protein synthesis inhibitor. Furthermore, treatment of cells with tert -butyl hydroperoxide ( tert -BHP), an oxidative promoter, decreased SMP30 expression and ERK1/2 phosphorylation, while EGCg treatment inhibited these effects. Male mice (7-week-old) were divided into 4 groups—Control (saline), tert -BHP (1.5 mmol/kg tert -BHP), EGCg tert -BHP (30 mg/kg/day of EGCg and 1.5 mmol/kg tert -BHP), and EGCg (30 mg/kg/day). After oral EGCg administration for 6 consecutive days, EGCg tert -BHP group mice were administered tert -BHP. The tert -BHP-administered mice showed decreased SMP30 expression in the liver and increased aspartate aminotransferase and alanine transaminase (hepatic injury marker enzymes) activities; however, EGCg treatment attenuated these changes. Thus, EGCg-induced SMP30 upregulation may alleviate tert -BHP-induced liver injury. The findings of this study offer new perspectives of the anti-ageing properties of EGCg.
