摘要：Microplastics (MPs) alter soil aggregation stability. However, studies have yet to determine whether these alterations further affect microbial community structures and diversities within different soil aggregates and whether they influence the responses of soil microbial structures and diversities to MPs in different aggregate fractions. In this study, long-term soil incubation experiments and soil fractionation were combined to investigate the effects of polyethylene microplastics (PE-MPs) on soil aggregate properties and microbial communities in soil aggregates with different particle sizes. Results showed that the existence of PE-MPs significantly reduced the physicochemical properties of soil aggregates, inhibited the activities of soil enzymes, and changed the richness and diversity of bacterial and fungal communities. Such variations exerted notable differences in soil aggregate levels. The response sensitivity of bacteria in the silt and clay fraction was higher than that in the macroaggregate fraction, but the response sensitivity of fungi in the macroaggregate fraction was higher than that in the silt and clay fraction. Relationships and path analysis between soil aggregate properties and microbial communities after PE-MPs addition were proposed. PE-MPs affected microbial community structures by directly and indirectly influencing soil microenvironmental conditions. The relative abundances of Acidobacteria , Gemmatimonadetes , Bacteroides , Basidiomycota , Chtridiomyota , and Glomeromycota were significantly correlated with physicochemical properties and soil enzyme activities. Enzyme activities were direct factors influencing soil microbial community structures, and physicochemical properties (i.e., dissolved organic carbon, soil available phosphorus) could indirectly affect these structures by acting on soil enzyme activities. Our findings helped improve our understanding of the responses of soil microbial structures and diversities to MPs through the perspective of different soil aggregates.
