摘要：Sweet cherries (Prunus avium) are rich in flavonoids such as anthocyanins that are beneficial for the immune system. The aim of the present study was to investigate in vitro antigenotoxicity and anticytotoxicity of total antochyanins extracted from Prunus avium (TAP) using by chromosomal aberration (CA), single cell gel electrophoresis (Comet) and micronucleus assay (MN) in human peripheral lymphocytes. 50, 100, 200 and 400 μg/mL concentrations of TAP was screened for its protective activity using mytomicin-C and H2O2 induced DNA damage in human lymphocytes. Also, positive, negative and solvent controls were used. According to the positive control TAP statistically decreased the abnormal cell percentage and CA/cell in 200 and 400 μg/mL at 24 h treatment and in all concentrations at 48 h. On the other hand, it was significantly increased the mitotic index in only 200 μg/mL at 24h and in all concentrations (except 200μg/mL) at 48h. Similarly, TAP were reduced MN frequency in all concentrations according to the positive control. In the comet assay, significant decreases in comet tail length, tail moment and tail intensity were observed in all concentrations. According to these results, we demonstrate that TAP has potent anticytotoxic and antigenotoxic effect against MMC and H2O2- induced genotoxicity.
其他摘要：Kiraz (Prunus avium) antosiyanin gibi immün sistem için faydalı olan flavonoidler açısından oldukça zengin bir bitkidir. Bu çalışmada amaç Prunus avium’dan ekstrakte edilen total antosiyaninlerin (TAP) in vitro antigenotoksik ve antisitotoksik etkilerini
