出版社：Korean Society of Environmental Health and Toxicology
摘要：Physiological cellular activities responses to cadmium (Cd) cxposure in green algae with several reductases activities and viability of the cell were examined. The cell division of green algac,Selenastrum capricorutum reated with Sppm was significantly decreased than that of normal algac. The mean cell number of normal algal culture was as lwice much as than that of alga at 6 days after Cd treatment. The ellular viability of algae was analysed by flow - cytometry with fluorescent dye after esterase reaction on cell membrane. The 85.35% of cellular viability of normal culture was decreased 1o 3435% when algat was treated with 5 ppm of Cd at 6 days after treatment. It was considered that those method of flow - cytometry is useful tool for toxicity test on micro - organisms in the respet of identifying cllular viability. Also,the activities of both glutathione perox- idase (GPX) and ascorbate peroxidase (APX). which are indirectly react against oxidative stress through reduc- tion of glutathione by Cd were signifcantly increased with 25%. It is considered that both GPX and APX are involved in the metabolic pathway of Cd- dtoxification with similar portion in Selenastrun capricormum.
关键词：cadmium toxicity. green algae. ellular vitality. peroxidase activities
