出版社：Korean Society of Environmental Health and Toxicology
摘要：In order to get insight into the mechanism of cadmium (Cd)-induced brain injury,we investigated the effects of Cd on the induction of COX-2 in bEnd.3 mouse brain endothelial cells. Cd induced COX-2 expression and PGE2 release,which were attenuated by thiol-reducing antioxidant N-acetylcysteine (NAC) indicating oxidative components might contribute to these events. Indeed,Cd increased cellular reactive oxygen species (ROS) level and DNA binding activity of nuclear factor-kB (NF-κB),an oxidative stress sensitive transcription factor. Cd-induced PGE2 production and COX-2 expression were significantly attenuated by Bay 11 7082,a specific inhibitor of NF-κB and by SB203580,a specific inhibitor of p38 mitogen activated protein kinase (MAPK). These data suggest that Cd induces COX-2 expression through activation of NF-κB and p38 MAPK, the oxidative stress-sensitive signaling molecules,in brain endothelial cells.
