出版社：Korean Society of Environmental Health and Toxicology
摘要：To investigate the posible enhancement of genotoxicity in stress environment,we examined the effect of genotoxic material in oxidative stress-induced condition using human cell line. Human lymphoblast cell line. TK6 was treated with hydrogen peroxide (H:O) for induction of oxidative stress,and treated with N-methyI- N'- nitroguanidine (MNNG),as a genetoxic material. We carried out MTS assay to set Ireatment doses. TK6 was treated with H2O: as 6.75 (low dose) or 13.5 μuM (high dose) for 2 h,and treated with MNNG as 0.1 17 (low dose),0.234 (middle dose). 0.468 μM high dose) for 2h. As results,a treatment of MNNG induced DNA dam- age as dose dependently. And TK6 treated with H:O2 at low as well as high dose followed by MNNG treal- ment showed higher DNA damage compared to MNNG alone treated groups. Malondialdehyde,as a marker of lipid peroxidation was increased in H/O;and MNNG treated groups. Real-time RT-PCR analyses for expres- sion of several antioxidative enzymes showed that catalase mRNA and glutathione peroxidase I mRNA expres- sion were decreased in H:Oz and MNNG treated groups. Taken together,we conclude that genotoxicity induced by MNNG is enhanced in a condition of oxidative stress induced by HO: and it suggests that it should be asso- ciated with induction of lipid peroxidation and decrease of antioxidant enzymes.
关键词：genotoxicity;oxidative sress;hydrogen peroxide (H2O2);N'-methyI-N"-nitroguanidine (MNNG); TK6 cell line
