出版社：Korean Society of Environmental Health and Toxicology
摘要：Toxicity of ocean dumping wastes (dye waste,urban sewage,food waste) were examined by observing fertil- ization and embryo development raltes of the Sea Urchin,Strongylocentrotus nudus. Spawning was induced by injecting I mL of 0.5 M KCl into coelomic cavity. Males released white or cream-colored sperms and females released yellow or orange -colored eggs. Experiments were began within 30 min after the ollction of both gametes. The ferilization and embryo development rates tests were performed for 10 min and 48 h after fertili- zation,respectively. The fertiliation and embryo developrment rates in the control condition (not including ocean dumping wastes sludge elutriate) were greater than 90%,but markedly decreased with increasing con- centrations of ocean dumping waste sludge elutriate. The fertilization and normal embryogenesis rates were significantly inhibited in all waste sludge elutriate from dye waste (ECgo=5.76;ECs0=4.53),urban sewage (ECso=9.82;EC%o=9.67) and food waste (ECso=3.90;ECso=3.27),respectively. The NOEC (> 3.13%) and LOEC(3.13%) of frilitin and normal embryogenesis rates very similar in all waste sludge elutriate. These results suggest biological assay using the fertilization and embryo development rates of S. mudus are very useful test method for the ecological toxicity asessment of ocean dumping wastes.
