出版社：Korean Society of Environmental Health and Toxicology
摘要：The purpose of this study is to evaluate the skin whitening effect of Scirpi rhizama ethanol extract (SREE) in an animal model. For the experiment of the study,three brown guinea pigs weighing about 450g to 550g were exposed to ultraviolet-B rays on the backs at 500 mJ/cm' once a week,three consecutive weeks and the total quantity of light was 1,500 m/cm2. The artificial tanning spots were divided into six diferent groups including normal (N),control (C),vehicle control (VC),positive control (PC),experimental 1 (E1,1% SREE),experimental 2(E2,2% SREE) groups. Then,30μL of SREE was transdermaly applied on the arificial tanning spots twice a day and 5 days a week for 8 weeks. With the result of a gross observation,it was found that the degree of pig- mentation became apparently thinner in the group applied with E2,compared to the control or the vehicle con- trol group. The melanin index of E2 group was signifiant lower than the control or the vehicle control group. In the observation with a light microscope,it was found that the degree of melanin pigmentation and S- 100 protein expression considerably decreased in the groups applied with SREE. compared to the control or the vehi- cle control group. With the numerical analysis of melanin pigmentation and S- 100 protein expression by using image analysis sofware. it was found that the tendency was coincide with the results of microscopic observation.
关键词：brown guinea pig;melanin index;melanin pigmentation;S- 100 protein
