出版社：Korean Society of Environmental Health and Toxicology
摘要：Plancodon granid/lorum. Doraji as Korean name,is one of the most widely used trditional oriental medicine for bronchial diseases and also used as a folk remedy for geriatic diseases and inlammatory diseases. In rcent studies,it has been reported that some efct of P. grandjfloruom is derived from is antioxidant activity although there is still a lack of evidence to establish its oxy-radical scavenging activity. In this study,total oxy-radical scavenging capacity (TOSC) assay was used to evaluate antioxidant activity of total extracts (T-PG),polysac- charide fraction (Po-PG),and saponin fraction (Sa-PG) isolated from P. grandiforun against peroxyI radicals and peroxynitrites. And MTT assay was taken to assess cyto-protective ffects of T-PG,Po-PG and Sa-PG in H4IIE cells treated with hydrogen peroxide and tert butyhydroperoxide. In the TOSC assay,Sa-PG showed strong oxy-radical scavenging eapacity compared with T PG and Po-PG. In cell-bused assay,T-PG and Po-PG proected cells from oxidative stress,but Sa-PG did not protect cells because of cytotoxicily of Sa-PG. These results suggest that the saponin components of P. graundijforum have relatively strong antioxidant capacity and cytoloxicity in rat hepatoma cells.
