出版社：Korean Society of Environmental Health and Toxicology
摘要：Crucian carp (Carassius auratus) has been used as the sentinel species for POPs (Persistent Organic Pol- lutants) monitoring in aquatice environment. However,there is lttle information for dioxin toxicity and espe- cially,early life stage toxicity in crucian carp have been never carried out. In this study,we investigated several toxic effects for 23,7 8-tetrachlorodibenzo-p-dioxin (2.3,7 8-TCDD) in ferilized egg obtained by natural frili- zation from crucian carp. The embryos at 3 h post- frilization (hpf) were treated with 0.039,0.156,0.625,and 2.5 (ug/L) TCDD by waterbormne exposure for 60 minutes and changed with fresh water 2 times per day. Ferti- lized eggs started hatching at 51 hpf and TCDD exposed embryo showed decrease of hatching rate in a dose- dependent manner at 75 hpf. Pericardial edema was continuously observed in larvae exposed to TCDD from hatching start time (51 hpf),followed by the onset of mortality. In addition,AhR-related gene,CYPIA was . clearly increased by TCDD in a dose dependent manner. These results indicated that ferilized eggs obtained from crucian carp have the TCDD related gene regulation and a distinct TCDD developmental toxicity syndrome by TCDD exposure. Therefore,we suggested that early life stage test in crucian carp could be used as test methods on dioxins toxicity.
关键词：Carassius auratus;Early life stage;TCDD developmental toxicity;CYPIA
