出版社：Korean Society of Environmental Health and Toxicology
摘要：Oxidative stress was evaluated for anthracene (Ant) and alkyl-Ants (9-methylanthracene [9-MA] and 9,10-dimethylanthracene [9,10- DMA]) in Caenorhabditis elegans to compare changes in toxicity due to the degree of alkylation. Worms were exposed at 1) the same external exposure concentration and 2) the maximum water-soluble concentration. Formation of reactive oxygen species,superoxide dismutase activity,total glutathione concentration,and lipid peroxidation were determined under constant exposure conditions using passive dosing. The expression of oxidative stress-related genes (daf-2,sir-2.1,daf-16,sod-1,sod-2,sod-3 and cytochrome 35A/C family genes) was also investigated to identify and compare changes in the genetic responses of C. elegans exposed to Ant and alkyl-Ant. At the same external concentration,9,10-DMA induced the greatest oxidative stress,as evidenced by all indicators,except for lipid peroxidation,followed by 9-MA and Ant. Interestingly,9,10-DMA led to greater oxidative stress than 9-MA and Ant when worms were exposed to the maximum water-soluble concentration,although the maximum water-soluble concentration of 9,10- DMA is the lowest. Increased oxidative stress by alkyl-Ants would be attributed to higher lipid-water partition coefficient and the π electron density in aromatic rings by alkyl substitution,although this supposition requires further confirmation.
