出版社：Korean Society of Environmental Health and Toxicology
摘要：Using mixed incubation of cultured endothelial cells,cultured fibroblasts,neutrophils activated with PMA and paraquat,the production of superoxide anion,H2〇2 and lipoxygenase metabolites (5-HETE and 12-HETE) of arachidonate was estimated. The results was as follows: 1. Neutrophils activated with PMA was produced superoxide anion,H2〇2 and lipoxygenase metabolites (5-HETE and 12-HETE) of arachidonate. 2. Fibroblasts did not alter the production of superoxide anion and H2O2 by neutrophils,it was markedly reduced by mixed incubation with endothelial cells. 3. Mixed incubation with endothelial cells significantly augmented the production of 5 or 12-HETEs,but fibroblasts did not. 4. Using mixed incubation of endothelial cells,fibroblasts,neutrophils and paraquat (50/ig/ml and 100/ig/ml),the production of superoxide anion,H2O2 and HETEs was significantly increased on both cells at low concentration (50^g/ml),but markedly reduced at high concentration (100사g/ml). 5. Paraquat showed concentration dependent effects on arachidonate lipoxygenase metabolism.
