出版社：Korean Society of Environmental Health and Toxicology
摘要：The tetrazdium dye,3~(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT),is reduced by live but not dead cell,and this reaction is used as the end point in a rapid drug screening assay. It can also be used for accurate determinations of drug sensitivity but only if a qualitative relationship is established between cell number and MTT-formazan production. Several conditions were examined to devise an in vitro assay method in primary cultured hepatocytes,such as optimum wavelength,optimal MTT concentration,optimal incubation time,and cell density.
