期刊名称：Acta Universitatis Cibiniensis. Series E: Food Technology
印刷版ISSN：2344-150X
出版年度：2020
卷号：24
期号：2
页码：186-194
DOI：10.2478/aucft-2020-0017
出版社：Walter de Gruyter GmbH
摘要：The present study is focused on the determination of optimized activity conditions of peroxidase (POD) of turnip ( Brassica rapa ). The enzyme is extracted from turnip with 0.5M Na 2 HPO 4 (pH7.0). Peroxidase activity is evaluated by monitoring the oxidation of guaiacol to tetraguaiacol in the presence of hydrogen peroxide (H 2 O 2 ). Before the optimization of peroxidase activity from a turnip by response surface methodology (RSM), a preliminary step is conducted for each studied parameter (pH, temperature, and NaCl concentration). Further, RSM following Box-Behnken design is used in order to refine the optimization of the conditions. Response surface analysis showed that parameter values with the highest peroxidase activity are 5.9, 29.8°C, and 1.07M for pH, temperature, and NaCl, respectively. Under these experimental conditions, peroxidase activity is found to be 4027.25U/mg. The obtained experimental value agrees with that of the calculated one, indicating the pertinence of the developed model for the optimization of peroxidase activity.
