摘要：Understanding the responses of the microbial community and greenhouse gas (GHG) emissions to the incorporation of different organic amendments is essential for their proper utilization. In this study, laboratory-incubated microcosm experiments were conducted to investigate the short-term effects of pine-wood biochar and lignitebased amendment on the microbial communities and GHG emissions from agricultural soil. Soils amended at five different application rates were incubated for 19 d under the conditions of 60% water-filled pore space and 25 ˚C. Microbial biomass in the amended soil after incubation was measured by the solid colony counting method, and the soil microbial diversity was assayed using a Biolog EcoPlate. The biochar and lignite-based amendment had distinct effects on the soil microbial communities and GHG emissions. The microbial community growth and utilization of C sources were improved by the biochar but restrained by the lignite-based amendment in most cases. The biochar and lignite-based amendment had a minor impact on methane emissions. Carbon dioxide emissions were promoted by the biochar and inhibited by the lignite-based amendment during the short-term incubation period. Nitrous oxide emissions decreased with the application rate of biochar but increased with the rate of lignite-based amendment. The addition of biochar at a rate of 3–4% and lignite-based amendment at a rate of <1% has the potential to improve soil quality. Salt leaching is required to avoid accumulation when the biochar and lignite-based amendments are applied. The findings can provide a reference for the application of biochar and lignite-based amendment in silt loam soil.
