期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2020
卷号：27
期号：3
页码：485-488
DOI：10.26444/aaem/126619
出版社：Institute of Agricultural Medicine in Lublin
摘要：INTRODUCTION AND OBJECTIVES :The parasite Cryptosporidium spp. is an intracellular protozoa which has a broad range of hosts and zoonotic potential. It presents a serious health risk for agricultural workers and veterinarians. The aim of the study was to identify the species and subtypes of Cryptosporidium occurring in a veterinary student who came into contact with calves on a farm. MATERIAL AND METHODS :The Ziehl-Neelsen staining technique was employed to confirm the presence of Cryptosporidium oocysts. ELISA test was applied to detect coproantigen in faecal specimens. Nested PCR was used to amplify a small ribosomal subunit (SSU rRNA) and sequencing of the GP60 gene served to identify the zoonotic subtypes. RESULTS :The nested PCR allowed to confirm the C. parvum species; subsequently, the IIdA15G1 zoonotic subtype was identified. CONCLUSIONS :This is the first confirmed case in Slovakia of human cryptosporidiosis caused by the unique subtype IIdA15G1.
