摘要：The impact of fullerene C 60 water soluble daughter molecules - fullerols C 60 (OH) 24 nanoparticles (FNP) on emerging (non-aflatoxin biosynthetic pathway) toxins production in mycelia and yeast extract sucrose (YES) media of A. flavus was investigated under growth conditions of 29 °C in the dark for a 168 h period. The FNP solution (10, 100 and 1000 ng mL -1 ) contained predominantly nanoparticles of 8 nm diameter and with zeta potential mean value of -33 mV. Ten emerging metabolites were produced at concentrations reaching 1,745,035 ng 50 mL -1 YES medium. Seven of the metabolites were found in mycelia and media, while three were only in mycelia. Majority of the metabolites were detected in higher quantity in mycelia than in media, at a ratio of 99:1 (m/m). However, higher metabolite quantities were found in media following FNP application, while FNP caused a decrease of total metabolite quantities in mycelia. The concentrations of the metabolites in media increased in the presence of 1000 ng mL -1 FNP while mycelial quantities of the metabolites decreased with increased applied FNP dose. The impacts of global climate changes on FNP availability in the environment and on mycotoxin occurrence in crops increase the relevance of this study for risk assessment of nanoparticles. Cordycepin is reported for the first time as metabolite of A. flavus.
