摘要：Recent research has identified a population of PD-1 hi CXCR5 - 'peripheral helper' T (Tph) cells that simulate plasma cell differentiation by interactions between IL-21 and SLAMF5. However, the alteration of circulating Tph and CD138 B in IgA nephropathy (IgAN) remains poorly understood. Flow cytometry analysis was used to measure the frequency of circulating PD-1 hi CXCR5 - T cells and CD138 B cells in 37 patients with IgAN and 23 healthy controls (HCs). Estimated glomerular filtration rate (eGFR), 24 h urinary protein and serum cytokine concentrations were measured. The percentage of different subsets of circulating PD-1 hi CXCR5 - T cells and CD138 B cells were significantly higher in patients with IgAN compared to HCs. Pretreatment, the percentage of different subsets of circulating PD-1 hi CXCR5 - T cells and CD138 B cells were negatively correlated with eGFR, the percentage of circulating CD138 B cells was positively correlated with 24-h urinary protein concentration, and the percentage of circulating PD-1 hi CXCR5 - , CD28 and ICOS T cells. Posttreatment, the percentage of different subsets of circulating PD-1 hi CXCR5 - T cells and CD138 B cells and serum IL-21 concentration were significantly reduced. Different subsets of circulating PD-1 hi CXCR5 - T cells contribute to the progression and pathogenesis of IgAN by regulating the differentiation of CD138 B cells through a combination of surface molecules.
