摘要：Osteoarthritis (OA), the most common joint disorder, is characterised by progressive structural changes in both the cartilage and the underlying subchondral bone. In late disease stages, subchondral bone sclerosis has been linked to heightened osteogenic commitment of bone marrow stromal cells (BMSCs). This study utilised cell sorting and immunohistochemistry to identify a phenotypically-distinct, osteogenically-committed BMSC subset in human OA trabecular bone. Femoral head trabecular bone tissue digests were sorted into CD45-CD271 CD56 CD146-, CD45-CD271 CD56-CD146 and CD45-CD271 CD56-CD146-(termed double-negative, DN) subsets, and CD45 CD271-hematopoietic-lineage cells served as control. Compared to the CD146 subset, the CD56 subset possessed a lower-level expression of adipocyte-associated genes and significantly over 100-fold higher-level expression of many osteoblast-related genes including osteopontin and osteocalcin, whilst the DN subset presented a transcriptionally ‘intermediate’ BMSC population. All subsets were tri-potential following culture-expansion and were present in control non-OA trabecular bone. However, while in non-OA bone CD56 cells only localised on the bone surface, in OA bone they were additionally present in the areas of new bone formation rich in osteoblasts and newly-embedded osteocytes. In summary, this study reveals a distinct osteogenically-committed CD271 CD56 BMSC subset and implicates it in subchondral bone sclerosis in hip OA. CD271 CD56 subset may represent a future therapeutic target for OA and other bone-associated pathologies.
