期刊名称：Computational and Structural Biotechnology Journal
印刷版ISSN：2001-0370
出版年度：2021
卷号：19
页码：1531-1540
DOI：10.1016/j.csbj.2021.03.007
出版社：Computational and Structural Biotechnology Journal
摘要：Macroalgae is regarded as a promising third-generation marine biomass that can be utilized as a sustainable feedstock for bio-industry due to the high sugar level and absence of lignin. Alginate, composed of 1,4-linked D-mannuronate (M) and L-guluronate (G), is one of the major carbohydrates in brown macroalgae. It is difficult to be assimilated by most industrial microorganisms. Therefore, developing engineered microorganisms that can utilize alginate as a feedstock in order to produce natural products from marine biomass is critical. In this study, we isolated, characterized, and sequenced Vibrio sp. SP1 which rapidly grows using alginate as a sole carbon source. We further engineered this strain by introducing genes encoding enzymes under the control of synthetic expression cassettes to produce lycopene and β-carotene which are attractive phytochemicals owing to the antioxidant property. We confirmed that the engineered Vibrio sp. SP1 could successfully produce 2.13 ± 0.37 mg L −1 of lycopene, 2.98 ± 0.43 mg L −1 of β -carotene, respectively, from 10 g L −1 of alginate as a sole carbon source. Furthermore, our engineered strain could directly convert 60 g L −1 of brown macroalgae Sargassum fusiforme into 1.23 ± 0.21 mg L −1 of lycopene without any pretreatment which had been vitally required for the previous productions. As the first demonstrated strain to produce high-value product from Sargassum , Vibrio sp. SP1 is evaluated to be a desirable platform for the brown macroalgae-based biorefinery.
