文章基本信息
- 标题：L-histidine and L-carnosine exert anti-brain aging effects in D-galactose-induced aged neuronal cells
- 本地全文：下载
- 作者：Yerin Kim ; Yuri Kim
- 期刊名称：Nutrition Research and Practice
- 印刷版ISSN：1976-1457
- 出版年度：2020
- 卷号：14
- 期号：3
- 页码：188-202
- DOI：10.4162/nrp.2020.14.3.188
- 出版社：KoreaMed Synapse
- 摘要：BACKGROUND/OBJECTIVES :Brain aging is a major risk factor for severe neurodegenerative diseases. Conversely, L-histidine and L-carnosine are known to exhibit neuroprotective effects. The aim of this study was to examine the potential for L-histidine, L-carnosine, and their combination to mediate anti-brain aging effects in neuronal cells subjected to D-galactose-induced aging. MATERIALS/METHODS :The neuroprotective potential of L-histidine, L-carnosine, and their combination was examined in a retinoic acid-induced neuronal differentiated SH-SY5Y cell line exposed to D-galactose (200 mM) for 48 h. Neuronal cell proliferation, differentiation, and expression of anti-oxidant enzymes and apoptosis markers were subsequently evaluated. RESULTS :Treatment with L-histidine (1 mM), L-carnosine (10 mM), or both for 48 h efficiently improved the proliferation, neurogenesis, and senescence of D-galactose-treated SH-SY5Y cells. In addition, protein expression levels of both neuronal markers (β tubulin-III and neurofilament heavy protein) and anti-oxidant enzymes, glutathione peroxidase-1 and superoxide dismutase-1 were up-regulated. Conversely, protein expression levels of amyloid β (1-42) and cleaved caspase-3 were down-regulated. Levels of mRNA for the pro-inflammatory cytokines, interleukin (IL)-8 , IL-1β , and tumor necrosis factor-α were also down-regulated. CONCLUSIONS :To the best of our knowledge, we provide the first evidence that L-histidine, L-carnosine, and their combination mediate anti-aging effects in a neuronal cell line subjected to D-galactose-induced aging. These results suggest the potential benefits of L-histidine and L-carnosine as anti-brain aging agents and they support further research of these amino acid molecules. ©2020 The Korean Nutrition Society and the Korean Society of Community Nutrition.
- 关键词：Histidine;aging;brain;carnosine;neurogenesis
