摘要：Turkey is one of the genetic centers of several plum species including, P. cerasifera, P. instita, P. domestica and P. spinosa. Plum species grown in Turkey has very diverse plant features varied between shrubs and large tree, spreading to upright tree form and diverse blooming time. In present study, genetic variation among 16 wild plum (P. divaricata) genotypes naturally grown around Erciyes mountain in Central Anatolia were investigated by using inter-simple sequence repeat (ISSR) markers. DNA was extracted from young leaves by the CTAB method. Fifteen ISSR primers produced clear fragments were used for the study. PCR reaction components, PCR cycling parameters, electrophoresis and gel imaging procedures were performed. A 100 bp standard DNA ladder was used for estimating sizes of fragments. Data of molecular analyses were performed as follows: Bands obtained from ISSR primers were scored based on their scorability. Cluster analysis was performed in accordance with unweighted pair group method with arithmetic averages (UPGMA) method and dendrogram was created with NTSYS pc 2.11 software. using 15 primers of 119 bands obtained, 102 were polymorphic (87.6%). The unweighted pair-group method arithmetic average analysis demonstrated that the genotypes had a similarity range from 0.66 to 0.89. High level of genetic diversity was observed among plum materials. This diversity may be due to seed based propagation of the genotypes. These wild plum genotypes can be used for the expansion of the gene pool and breeding studies.
