文章基本信息

标题：Aerobic training-induced upregulation of YAP1 and prevention of cardiac pathological hypertrophy in male rats
本地全文：下载
作者：Arezoo Tabrizi ; Rahman Soori ; Siroos Choobineh 等
期刊名称：INTERNATIONAL JOURNAL OF PREVENTIVE MEDICINE
印刷版ISSN：2008-7802
出版年度：2020
卷号：11
期号：1
页码：119-123
DOI：10.4103/ijpvm.IJPVM_356_19
出版社：ISFAHAN UNIVERSITY OF MEDICAL SCIENCES
摘要：Background :Pathological hypertrophy is one of the negative consequences of cardiac sympathetic hyperactivity. Recent studies have shown that YAP1 plays a critical role in cardiomyocytes hypertrophy. Considering the preventive role of exercise training in cardiovascular diseases, the present study was conducted to examine the effect of aerobic exercise training on YAP1 gene expression and its upstream components. Methods :Eighteen male Wistar rats were randomly divided into aerobic training and control groups. Aerobic training was performed one hour/day, five days per week, for eight weeks, on a treadmill at 65-75% VO 2 max. Pathological hypertrophy was induced by injecting 3 mg/kg -1 of isoproterenol for seven days. The left ventricle was separated, and YAP1, 3-mercaptopyruvate sulfurtransferase (MST), large tumor suppressor (LATS), and mitogen-activated protein 4 kinase (MAP4K) gene expressions were assessed and YAP1 protein levels were also assessed by western blotting. Cell apoptosis was detected by TUNEL assays. The between-group differences were evaluated using the T-test and P value <0.05 was considered statistically significant. Results :There were no significant between-group differences in MST gene expression ( P = 0.061); meanwhile, in the training group, LATS and Map4K expressions were suppressed, followed by a significant increase in YAP1 expression ( P < 0.001). Compared to the control group, the left ventricular weight increased significantly in the training group while the cardiomyocyte apoptosis decreased. Conclusions :The results showed that, by reducing LATS, aerobic training-induced YAP1 upregulation can help prevent the propagation of cardiomyocyte apoptosis due to pathological conditions. Copyright: © 2020 International Journal of Preventive Medicine.
关键词：Aerobic exercise;YES associated protein1;cardiac hypertrophy;large tumor suppressor;mammalian sterile 20 like kinase