摘要：Ruminants are the main source of methane emissions from the agricultural sector. Emission inventories and mitigation strategies require reliable technics of measurement. The respiration chamber methodology is a precise approach for measuring enteric methane emissions in cattle. A set of experiments was carried out to validate two respiration chambers for measuring enteric methane emissions of cattle. The chambers were calibrated considering three main components: the methane analyzer, the air duct and air extraction system, and the chamber itself, by evaluating linearity and response time of the analyzer, plateau variability and high purity methane recoveries in chambers. Recovery test calculations carried out after releasing high purity methane into the chambers gave calibration factors of 0.95 ± 0.05 and 1.03 ± 0.03 for the complete system of respirations chambers one and two, respectively, with corresponding uncertainties of 4.87 and 2.49%. Thus, the respiration chambers for enteric methane measurements of cattle at the University of Yucatan, Mexico function with precision and accuracy. This technique can be used to establish methane inventories and methane mitigation strategies in cattle.
