首页    期刊浏览 2024年10月07日 星期一
登录注册

文章基本信息

  • 标题:Phase transition of RNA−protein complexes into ordered hollow condensates
  • 本地全文:下载
  • 作者:Ibraheem Alshareedah ; Mahdi Muhammad Moosa ; Muralikrishna Raju
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:2020
  • 卷号:117
  • 期号:27
  • 页码:15650-15658
  • DOI:10.1073/pnas.1922365117
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Liquid−liquid phase separation of multivalent intrinsically disordered protein−RNA complexes is ubiquitous in both natural and biomimetic systems. So far, isotropic liquid droplets are the most commonly observed topology of RNA−protein condensates in experiments and simulations. Here, by systematically studying the phase behavior of RNA−protein complexes across varied mixture compositions, we report a hollow vesicle-like condensate phase of nucleoprotein assemblies that is distinct from RNA−protein droplets. We show that these vesicular condensates are stable at specific mixture compositions and concentration regimes within the phase diagram and are formed through the phase separation of anisotropic protein−RNA complexes. Similar to membranes composed of amphiphilic lipids, these nucleoprotein−RNA vesicular membranes exhibit local ordering, size-dependent permeability, and selective encapsulation capacity without sacrificing their dynamic formation and dissolution in response to physicochemical stimuli. Our findings suggest that protein−RNA complexes can robustly create lipid-free vesicle-like enclosures by phase separation.
  • 关键词:RNA vesicles ; biomolecular condensates ; MD simulation ; optical tweezer ; nucleoprotein assembly
国家哲学社会科学文献中心版权所有