期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2020
卷号：117
期号：21
页码：11836-11842
DOI：10.1073/pnas.1918068117
出版社：The National Academy of Sciences of the United States of America
摘要：Systematic mappings of protein interactome networks have provided invaluable functional information for numerous model organisms. Here we develop P CR-mediated L inkage of barcoded A dapters T o nucleic acid E lements for seq uencing (PLATE-seq) that serves as a general tool to rapidly sequence thousands of DNA elements. We validate its utility by generating the ORFeome for Oryza sativa covering 2,300 genes and constructing a high-quality protein–protein interactome map consisting of 322 interactions between 289 proteins, expanding the known interactions in rice by roughly 50%. Our work paves the way for high-throughput profiling of protein–protein interactions in a wide range of organisms.
