期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2021
卷号：118
期号：14
页码：1
DOI：10.1073/pnas.1920827118
出版社：The National Academy of Sciences of the United States of America
摘要：In nerve cells the genes encoding for α 2 δ subunits of voltage-gated calcium channels have been linked to synaptic functions and neurological disease. Here we show that α 2 δ subunits are essential for the formation and organization of glutamatergic synapses. Using a cellular α 2 δ subunit triple-knockout/knockdown model, we demonstrate a failure in presynaptic differentiation evidenced by defective presynaptic calcium channel clustering and calcium influx, smaller presynaptic active zones, and a strongly reduced accumulation of presynaptic vesicle-associated proteins (synapsin and vGLUT). The presynaptic defect is associated with the downscaling of postsynaptic AMPA receptors and the postsynaptic density. The role of α 2 δ isoforms as synaptic organizers is highly redundant, as each individual α 2 δ isoform can rescue presynaptic calcium channel trafficking and expression of synaptic proteins. Moreover, α 2 δ-2 and α 2 δ-3 with mutated metal ion-dependent adhesion sites can fully rescue presynaptic synapsin expression but only partially calcium channel trafficking, suggesting that the regulatory role of α 2 δ subunits is independent from its role as a calcium channel subunit. Our findings influence the current view on excitatory synapse formation. First, our study suggests that postsynaptic differentiation is secondary to presynaptic differentiation. Second, the dependence of presynaptic differentiation on α 2 δ implicates α 2 δ subunits as potential nucleation points for the organization of synapses. Finally, our results suggest that α 2 δ subunits act as transsynaptic organizers of glutamatergic synapses, thereby aligning the synaptic active zone with the postsynaptic density.
