摘要：A methanol extract from the underground part of Calanthe discolor Lindl. (Orchidaceae) demonstrated significant proliferative activity on human hair follicle dermal papilla cells (HFDPC, % of control: 120.8 ± 0.2%) at 100 µg/mL against HFDPC. Through bioassay-guided separation of the extract, a new indole glycoside named 6′- O -β-D-apiofuranosylindican ( 1 ) was isolated along with six known compounds ( 2 – 7 ) including three indole glycosides. The stereostructure of 1 was elucidated based on its spectroscopic properties and chemical characteristics. Among the isolates, 1 (110.0 ± 1.0%), glucoindican ( 3 , 123.9 ± 6.8%), and calanthoside ( 4 , 158.6 ± 7.1%) showed significant proliferative activity at 100 µM. Furthermore, the active indole glycosides ( 1 , 3 , and 4 ) upregulated the expression of vascular endothelial growth factor (VEGF) and fibroblast growth factor-7 (FGF-7) mRNA and protein in HFDPC, which could be the mechanism of their proliferative activity.
