期刊名称：Chemical Industry and Chemical Engineering Quarterly
印刷版ISSN：1451-9372
出版年度：2013
卷号：19
期号：2
页码：221-229
DOI：10.2298/CICEQ120320056C
出版社：Association of the Chemical Engineers
摘要：One of the most important crops used for feeding of animals is maize (Zea mays L). Kernels of maize contain 7-13 g of protein/100 g of dry matter, and they are protagonist of chemical and physical changes during the processing of raw material. There are several methods for determination of protein changes during thermal processing. The objective of this study was to investigate application of Lab-on-a-Chip electrophoresis as a method for qualitative and quantitative determination of maize protein modifications during pelleting. Parameters which were varied during pelleting process were diameter of sieve openings of the hammer mill and duration of steam conditioning process. According to the results, conditioning retention time of 5 min could be considered as mild in terms of protein changes for samples ground to pass 3 and 4 mm sieve. Conditioning retention time of 10 min was long enough for achieving of high level of protein modifications for all samples. Regression analysis of obtained data showed that both independent parameters had significant (p < 0.05) influence on response variable (protein solubility). Protein electrophoregrams of investigated samples were qualitatively nearly identical, but quantitative differences occurred, especially in the range 18-85 kDa of protein molecular weight (MW). Results in this paper have proven that Lab-on-a-Chip electrophoresis registered changes in protein structure even when protein solubility was not changed, unlike other conventional methods. This method can be used for determination of degree of protein changes during pelleting process with remarkable certainty.
关键词：pelleting; long-term conditioning; protein changes; Lab-on-a-Chip electrophoresis
